TonB-Energiesystem

Net emissions of CO 2 by human activities—including not only energy services and industrial production but also land use and agriculture—must approach zero in order to stabilize global mean temperature. Energy services such as light-duty transportation, heating, cooling, and lighting may be relatively straightforward to decarbonize by electrifying and

What is the difference between ExbB and TonB?

PowerPoint slide ExbB is predicted to contain three transmembrane spanning helices (TMHs) and a large cytoplasmic domain, whereas ExbD and TonB are each predicted to contain a single N-terminal TMH that anchors a large C-terminal periplasmic domain in the inner membrane 11, 12, 13, 14, 15 (Fig. 1a).

What are the components of a ton system?

The Ton system consists of two elements: an energy-transducing Ton complex located within the inner membrane, and a ligand-specific TonB-dependent transporter within the outer membrane, which interacts with the ligands to be transported 2, 7, 8, 9 (Fig. 1a).

Are Tonb systems involved in the uptake and catabolism of aromatic compounds?

Although a number of TonB systems involved in the uptake of siderophores, vitamin B12 and saccharides have been identified, their involvement in the uptake and catabolism of aromatic compounds was previously unknown.

What is the function of the ton complex?

The Ton complex relies on the PMF for its function 27, 33 and it has been proposed that the Ton complex acts as a proton-conducting channel that shuttles protons from the periplasm to the cytoplasm and that this powers a mechanical motion within the complex 20.

Which periplasmic domain contains two stages in Tonb energization?

Ollis, A. A., Kumar, A. & Postle, K. The ExbD periplasmic domain contains distinct functional regions for two stages in TonB energization. J. Bacteriol. 194, 3069–3077 (2012) Ollis, A. A. & Postle, K. ExbD mutants define initial stages in TonB energization. J. Mol. Biol. 415, 237–247 (2012)

What is the conductance of a ton subcomplex compared to a ExbB pentamer?

Single- and multichannel recordings revealed that channels formed by the Ton subcomplex display a conductance of 120 ± 30 pS at neutral pH (Fig. 5a, b), whereas channels formed by the ExbB pentamer are nearly twice as large with a conductance of 220 ± 50 pS (Fig. 5b).